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A novel cold-adapted beta-galactosidase from Alteromonas sp. ML117 cleaves milk lactose effectively at low temperature
文献类型:期刊论文
作者:Yao, Congyu[1]  Sun, Jingjing[2]  Wang, Wei[3]  Zhuang, Zhiwei[4]  Liu, Junzhong[5]  Hao, Jianhua[6]  
机构排名:[1]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.;Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China.;
[2]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.;Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China.;
[3]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Shandong, Peoples R China.;
[4]New Hope Liuhe Co Ltd, Qingdao 266071, Shandong, Peoples R China.;
[5]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Shandong, Peoples R China.;
[6]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Shandong, Peoples R China.;Jiangsu Collaborat Innovat Ctr Exploitat & Utiliz, Lianyungang 222005, Peoples R China.;
通讯作者:Hao, JH (reprint author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China.; Hao, JH (reprint author), Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Shandong, Peoples R China.; Hao, JH (reprint author), Jiangsu Collaborat Innovat Ctr Exploitat & Utiliz, Lianyungang 222005, Peoples R China.
年:2019
期刊名称:PROCESS BIOCHEMISTRY影响因子和分区
卷:82
页码范围:94-101
增刊:正刊
收录情况:SCI(E)(WOS:000470942900011)  
所属部门:黄海水产研究所;海洋生物酶工程创新团队
语言:外文
ISSN:1359-5113
被引频次:8
人气指数:486
浏览次数:486
基金:Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS [20603022018006]; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology [2016ASKJ14]
关键词:Alteromonas; Cold-adapted enzyme; beta-galactosidase; Enzyme properties; Lactose hydrolysis
摘要:
A novel beta-galactosidase gene (Bgal) was cloned from the marine bacterium Alteromonas sp. ML117. Bgal from Alteromonas sp. ML117 was heterologously expressed in Escherichia coli BL21 (DE3) cells to study the enzymatic properties of the recombinant beta-galactosidase. Using gel-filtration chromatography and SDS-PAGE, the recombinant enzyme was identified to be a tetrameric enzyme that belonged to the glycoside hydrolase family GH2. The purified recombinant Bgal was able to hydrolyze lactose and ...More
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